Rapid purification of the peptide toxins microcystin-LR and nodularin

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منابع مشابه

On the Chemistry, Toxicology and Genetics of the Cyanobacterial Toxins, Microcystin, Nodularin, Saxitoxin and Cylindrospermopsin

The cyanobacteria or "blue-green algae", as they are commonly termed, comprise a diverse group of oxygenic photosynthetic bacteria that inhabit a wide range of aquatic and terrestrial environments, and display incredible morphological diversity. Many aquatic, bloom-forming species of cyanobacteria are capable of producing biologically active secondary metabolites, which are highly toxic to huma...

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Stability of cyanotoxins, microcystin-LR, microcystin-RR and nodularin in seawater and BG-11 medium of different salinity

Microcystins and nodularin are potent hepatotoxins produced by fresh and seawater cyanobacteria. The persistence of three hepatotoxins – microcystin-LR, microcystin-RR and nodularin – was investigated in sterile BG-11 medium of different salinity and in water collected from the Gulf of Gdańsk. After 21 days of incubation at 17± 1◦C and constant illumination of about 40 μmol photon m−2 s−1 the c...

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Screening of certain chemoprotectants against cyclic peptide toxin microcystin-LR

Objective: To evaluate the protective efficacy of certain chemoprotectants against cyclic peptide hepatotoxic microcystin-LR in mice. Material and Methods: Swiss albino female mice were used in all experiments for screening antidotes against the lethal dose of microcystin-LR (100 μg/kg body weight, i.p.). The agents, Dglucose, mannitol, dihydroxyacetone, Trolox, L-cysteine, N-acetylcysteine, am...

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Analysis of microcystin-LR and nodularin using triple quad liquid chromatography-tandem mass spectrometry and histopathology in experimental fish.

Microcystins (MCs) are hepatotoxic cyanobacterial metabolites produced sporadically in aquatic environments under favorable environmental conditions. Affinity of these toxins to covalently bind with protein phosphatases poses a challenge in their detection. Lemieux oxidation to release 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB), a common moiety to all MCs congeners, has been used in detecti...

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Colorimetric immuno-protein phosphatase inhibition assay for specific detection of microcystins and nodularins of cyanobacteria.

A novel immunoassay was developed for specific detection of cyanobacterial cyclic peptide hepatotoxins which inhibit protein phosphatases. Immunoassay methods currently used for microcystin and nodularin detection and analysis do not provide information on the toxicity of microcystin and/or nodularin variants. Furthermore, protein phosphatase inhibition-based assays for these toxins are not spe...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1990

ISSN: 0378-1097

DOI: 10.1111/j.1574-6968.1990.tb04112.x